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cells  (Miltenyi Biotec)


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    Miltenyi Biotec cells
    Cells, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 513 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd11b+cells/CD11b+MicroBeads%2C+human+and+mouse/pm42468469-103-3-9
    Average 96 stars, based on 513 article reviews
    cells - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Centrifugation:

    Article Title: Viral Microglia Reprogramming Clears Oligomeric Neurotoxic Debris
    Article Snippet: The pellet was resuspended in MACS Buffer (1 x PBS, 1 mM EDTA, 1% BSA) containing CD11b + MicroBeads (Miltenyi Biotec #130-093-634; 10 μl beads per 90 μl buffer) and incubated at 4°C (15 min). .. After rinsing cells with centrifugation at 300 x g (10 min, 4°C), CD11b + cells were isolated via LS magnetic columns (Miltenyi #130-042-401) mounted on a MACS Separator (Miltenyi #130-090-976). ..

    Isolation:

    Article Title: Viral Microglia Reprogramming Clears Oligomeric Neurotoxic Debris
    Article Snippet: The pellet was resuspended in MACS Buffer (1 x PBS, 1 mM EDTA, 1% BSA) containing CD11b + MicroBeads (Miltenyi Biotec #130-093-634; 10 μl beads per 90 μl buffer) and incubated at 4°C (15 min). .. After rinsing cells with centrifugation at 300 x g (10 min, 4°C), CD11b + cells were isolated via LS magnetic columns (Miltenyi #130-042-401) mounted on a MACS Separator (Miltenyi #130-090-976). ..

    Magnetic Cell Separation:

    Article Title: Viral Microglia Reprogramming Clears Oligomeric Neurotoxic Debris
    Article Snippet: The pellet was resuspended in MACS Buffer (1 x PBS, 1 mM EDTA, 1% BSA) containing CD11b + MicroBeads (Miltenyi Biotec #130-093-634; 10 μl beads per 90 μl buffer) and incubated at 4°C (15 min). .. After rinsing cells with centrifugation at 300 x g (10 min, 4°C), CD11b + cells were isolated via LS magnetic columns (Miltenyi #130-042-401) mounted on a MACS Separator (Miltenyi #130-090-976). ..

    Article Title: Targeting USP14 enhances immunotherapy response by reprogramming tumor-associated macrophages in colon cancer
    Article Snippet: .. For RNA and protein extraction, CD11b + cells were first enriched using magnetic-activated cell sorting (MACS) with anti-CD11b microbeads (Miltenyi Biotec) according to the manufacturer’s protocol. .. For functional assays requiring high purity, TAMs (defined as live CD45 + CD11b + F4/80 + cells) were isolated by fluorescence-activated cell sorting (FACS) using a BD FACSAria III cell sorter (BD Biosciences).

    Article Title: Old Blood, Young Bones: Identification of Middle‐Aged Myeloid Cells That Limit Cortical Bone Loss
    Article Snippet: .. To isolate CD11B + cells, cells were then suspended in 1× MACS Buffer and incubated with phycoerythrin (PE)‐conjugated anti‐CD11B (Miltenyi Biotec, #130–113‐235). .. Cells were washed, suspended in MACS buffer, and incubated with anti‐PE microbeads (Miltenyi Biotec, #130–048‐801).

    Article Title: Dual role of Ninjurin-1 in myeloid cell adhesion and inflammation in relapse-remitting EAE.
    Article Snippet: .. CD11b+ cells were enriched from naïve SJL/J splenocytes using MACS Magnetic separation columns (Miltenyi Biotec) and were plated at 0.5 x 106 cells/well in 24 well plates. ..

    Article Title: Dual role of Ninjurin-1 in myeloid cell adhesion and inflammation in relapse-remitting EAE
    Article Snippet: .. CD11b + cells were enriched from naïve SJL/J splenocytes using MACS Magnetic separation columns (Miltenyi Biotec) and were plated at 0.5 x 10 6 cells/well in 24 well plates. ..

    Article Title: Single‐Cell Nanodroplet Processing Proteomics Pipeline for Analysis of Human‐Derived Microglia
    Article Snippet: .. The CD11b+ cells were purified on an LS MACS column (Miltenyi, Gladbach, Germany) attached to a QuadroMACS separator (Miltenyi, Gladbach, Germany). ..

    Article Title: Claudin 1–mediated positioning of DC1 to mTECs is essential for maintenance of central tolerance
    Article Snippet: .. Next, cells were stained with anti-biotin magnetic beads (Miltenyi) according to the manufacturer’s protocol and CD11c + and CD11b + cells were MACS-enriched using QuadroMACS (Miltenyi). ..

    Protein Extraction:

    Article Title: Targeting USP14 enhances immunotherapy response by reprogramming tumor-associated macrophages in colon cancer
    Article Snippet: .. For RNA and protein extraction, CD11b + cells were first enriched using magnetic-activated cell sorting (MACS) with anti-CD11b microbeads (Miltenyi Biotec) according to the manufacturer’s protocol. .. For functional assays requiring high purity, TAMs (defined as live CD45 + CD11b + F4/80 + cells) were isolated by fluorescence-activated cell sorting (FACS) using a BD FACSAria III cell sorter (BD Biosciences).

    FACS:

    Article Title: Targeting USP14 enhances immunotherapy response by reprogramming tumor-associated macrophages in colon cancer
    Article Snippet: .. For RNA and protein extraction, CD11b + cells were first enriched using magnetic-activated cell sorting (MACS) with anti-CD11b microbeads (Miltenyi Biotec) according to the manufacturer’s protocol. .. For functional assays requiring high purity, TAMs (defined as live CD45 + CD11b + F4/80 + cells) were isolated by fluorescence-activated cell sorting (FACS) using a BD FACSAria III cell sorter (BD Biosciences).

    Incubation:

    Article Title: Old Blood, Young Bones: Identification of Middle‐Aged Myeloid Cells That Limit Cortical Bone Loss
    Article Snippet: .. To isolate CD11B + cells, cells were then suspended in 1× MACS Buffer and incubated with phycoerythrin (PE)‐conjugated anti‐CD11B (Miltenyi Biotec, #130–113‐235). .. Cells were washed, suspended in MACS buffer, and incubated with anti‐PE microbeads (Miltenyi Biotec, #130–048‐801).

    Purification:

    Article Title: Single‐Cell Nanodroplet Processing Proteomics Pipeline for Analysis of Human‐Derived Microglia
    Article Snippet: .. The CD11b+ cells were purified on an LS MACS column (Miltenyi, Gladbach, Germany) attached to a QuadroMACS separator (Miltenyi, Gladbach, Germany). ..

    Staining:

    Article Title: Claudin 1–mediated positioning of DC1 to mTECs is essential for maintenance of central tolerance
    Article Snippet: .. Next, cells were stained with anti-biotin magnetic beads (Miltenyi) according to the manufacturer’s protocol and CD11c + and CD11b + cells were MACS-enriched using QuadroMACS (Miltenyi). ..

    Magnetic Beads:

    Article Title: Claudin 1–mediated positioning of DC1 to mTECs is essential for maintenance of central tolerance
    Article Snippet: .. Next, cells were stained with anti-biotin magnetic beads (Miltenyi) according to the manufacturer’s protocol and CD11c + and CD11b + cells were MACS-enriched using QuadroMACS (Miltenyi). ..



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    Targeting USP14 inhibits tumor growth and induces local anti-tumor immunity in vivo (A) Representative images of MC38 tumors harvested on day 21 post-inoculation from mice treated with IU1 (20 mg/kg, i.p., on days 6, 9, 12, and 15) or vehicle control. (B) Statistics of MC38 tumor growth rate following IU1 or vehicle treatment in vivo . The intraperitoneal injection dose of IU1 was 20 mg/kg, administered on days 6, 9, 12, and 15. Data are presented as the mean ± SEM ( n = 6 per group). (C) Spider diagram of the tumor volume growth in each mouse from the IU1 group and the PBS group. (D) Gating strategy for the detection of the TAMs by flow cytometry. We first obtained live cells, and then identified cells that were positive for CD45, <t>CD11b,</t> F4-80, and CD206 as M2 macrophages. (E–P) Proportions of neutrophil (E), M2 macrophage (F), M1 macrophage (G), MDSC (H), activated DCs (I), CD4 T cell (J), Treg cells (K), CD8 T cell (L), IFN-γ + CD8 T cell (M), precursor exhausted T cells (TCF-1 + ) (N), effective CD8 T cells (PD-1 + ) (O) and activated CD8 T cell (CD69 + ) (P) in the TME of the IU1 group and the control group by using flow cytometry. (Q–U) Cytokines IFN-γ (Q), TNF-α (R), IL-2 (S), IL-10 (T), and IL-12 (U) in the TME of each group were detected by Mul-Analyte Flow Assay Kit. (V) Schematic illustration of the proposed mechanism of action of IU1 in reprogramming the tumor microenvironment. Data are presented as the mean ± SEM. ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001, and ns: not significant.
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    Image Search Results


    Targeting USP14 inhibits tumor growth and induces local anti-tumor immunity in vivo (A) Representative images of MC38 tumors harvested on day 21 post-inoculation from mice treated with IU1 (20 mg/kg, i.p., on days 6, 9, 12, and 15) or vehicle control. (B) Statistics of MC38 tumor growth rate following IU1 or vehicle treatment in vivo . The intraperitoneal injection dose of IU1 was 20 mg/kg, administered on days 6, 9, 12, and 15. Data are presented as the mean ± SEM ( n = 6 per group). (C) Spider diagram of the tumor volume growth in each mouse from the IU1 group and the PBS group. (D) Gating strategy for the detection of the TAMs by flow cytometry. We first obtained live cells, and then identified cells that were positive for CD45, CD11b, F4-80, and CD206 as M2 macrophages. (E–P) Proportions of neutrophil (E), M2 macrophage (F), M1 macrophage (G), MDSC (H), activated DCs (I), CD4 T cell (J), Treg cells (K), CD8 T cell (L), IFN-γ + CD8 T cell (M), precursor exhausted T cells (TCF-1 + ) (N), effective CD8 T cells (PD-1 + ) (O) and activated CD8 T cell (CD69 + ) (P) in the TME of the IU1 group and the control group by using flow cytometry. (Q–U) Cytokines IFN-γ (Q), TNF-α (R), IL-2 (S), IL-10 (T), and IL-12 (U) in the TME of each group were detected by Mul-Analyte Flow Assay Kit. (V) Schematic illustration of the proposed mechanism of action of IU1 in reprogramming the tumor microenvironment. Data are presented as the mean ± SEM. ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001, and ns: not significant.

    Journal: iScience

    Article Title: Targeting USP14 enhances immunotherapy response by reprogramming tumor-associated macrophages in colon cancer

    doi: 10.1016/j.isci.2026.115362

    Figure Lengend Snippet: Targeting USP14 inhibits tumor growth and induces local anti-tumor immunity in vivo (A) Representative images of MC38 tumors harvested on day 21 post-inoculation from mice treated with IU1 (20 mg/kg, i.p., on days 6, 9, 12, and 15) or vehicle control. (B) Statistics of MC38 tumor growth rate following IU1 or vehicle treatment in vivo . The intraperitoneal injection dose of IU1 was 20 mg/kg, administered on days 6, 9, 12, and 15. Data are presented as the mean ± SEM ( n = 6 per group). (C) Spider diagram of the tumor volume growth in each mouse from the IU1 group and the PBS group. (D) Gating strategy for the detection of the TAMs by flow cytometry. We first obtained live cells, and then identified cells that were positive for CD45, CD11b, F4-80, and CD206 as M2 macrophages. (E–P) Proportions of neutrophil (E), M2 macrophage (F), M1 macrophage (G), MDSC (H), activated DCs (I), CD4 T cell (J), Treg cells (K), CD8 T cell (L), IFN-γ + CD8 T cell (M), precursor exhausted T cells (TCF-1 + ) (N), effective CD8 T cells (PD-1 + ) (O) and activated CD8 T cell (CD69 + ) (P) in the TME of the IU1 group and the control group by using flow cytometry. (Q–U) Cytokines IFN-γ (Q), TNF-α (R), IL-2 (S), IL-10 (T), and IL-12 (U) in the TME of each group were detected by Mul-Analyte Flow Assay Kit. (V) Schematic illustration of the proposed mechanism of action of IU1 in reprogramming the tumor microenvironment. Data are presented as the mean ± SEM. ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001, and ns: not significant.

    Article Snippet: For RNA and protein extraction, CD11b + cells were first enriched using magnetic-activated cell sorting (MACS) with anti-CD11b microbeads (Miltenyi Biotec) according to the manufacturer’s protocol.

    Techniques: In Vivo, Control, Injection, Flow Cytometry