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cd11b cells  (Miltenyi Biotec)


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    Miltenyi Biotec cd11b cells
    Claudin 1 is upregulated in CAT-experienced DC1s. (A) FACS gating strategy used to perform scRNAseq of thymic myeloid cells. Thymic cells were isolated from Foxn1 Cre R26 TdTOMATO mice, MACS-enriched for CD11c + and <t>CD11b</t> + cells, and sorted as either TdTOMATO + or TdTOMATO−CD45 + CD11c + /CD11b + cells. (B) UMAP of annotated thymic myeloid cells from scRNAseq excluding subsets shown in red in the legend of . Individual cell lineages are demarcated by dotted lines. DC = conventional DC; aDC = activated DC; Mac = macrophages; Mono = monocytes; prolif = proliferating. (C) UMAP of scRNAseq corresponding to projecting CAT-experienced (orange) and CAT-inexperienced (gray) cells. A dotted line shows the DC1 subset. (D) Heat map showing top 10 down- and upregulated genes in CAT-experienced over CAT-inexperienced DC1s. The heat map color scale depicts average log2 fold change. (E) Violin plots show the expression of Cldn1 and Cd36 by CAT-experienced (orange) and CAT-inexperienced (gray) DC1. All mice were bred on the B6 background. Littermates were used.
    Cd11b Cells, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 99/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cd11b cells/product/Miltenyi Biotec
    Average 99 stars, based on 17 article reviews
    cd11b cells - by Bioz Stars, 2026-03
    99/100 stars

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    1) Product Images from "Claudin 1–mediated positioning of DC1 to mTECs is essential for maintenance of central tolerance"

    Article Title: Claudin 1–mediated positioning of DC1 to mTECs is essential for maintenance of central tolerance

    Journal: The Journal of Experimental Medicine

    doi: 10.1084/jem.20250970

    Claudin 1 is upregulated in CAT-experienced DC1s. (A) FACS gating strategy used to perform scRNAseq of thymic myeloid cells. Thymic cells were isolated from Foxn1 Cre R26 TdTOMATO mice, MACS-enriched for CD11c + and CD11b + cells, and sorted as either TdTOMATO + or TdTOMATO−CD45 + CD11c + /CD11b + cells. (B) UMAP of annotated thymic myeloid cells from scRNAseq excluding subsets shown in red in the legend of . Individual cell lineages are demarcated by dotted lines. DC = conventional DC; aDC = activated DC; Mac = macrophages; Mono = monocytes; prolif = proliferating. (C) UMAP of scRNAseq corresponding to projecting CAT-experienced (orange) and CAT-inexperienced (gray) cells. A dotted line shows the DC1 subset. (D) Heat map showing top 10 down- and upregulated genes in CAT-experienced over CAT-inexperienced DC1s. The heat map color scale depicts average log2 fold change. (E) Violin plots show the expression of Cldn1 and Cd36 by CAT-experienced (orange) and CAT-inexperienced (gray) DC1. All mice were bred on the B6 background. Littermates were used.
    Figure Legend Snippet: Claudin 1 is upregulated in CAT-experienced DC1s. (A) FACS gating strategy used to perform scRNAseq of thymic myeloid cells. Thymic cells were isolated from Foxn1 Cre R26 TdTOMATO mice, MACS-enriched for CD11c + and CD11b + cells, and sorted as either TdTOMATO + or TdTOMATO−CD45 + CD11c + /CD11b + cells. (B) UMAP of annotated thymic myeloid cells from scRNAseq excluding subsets shown in red in the legend of . Individual cell lineages are demarcated by dotted lines. DC = conventional DC; aDC = activated DC; Mac = macrophages; Mono = monocytes; prolif = proliferating. (C) UMAP of scRNAseq corresponding to projecting CAT-experienced (orange) and CAT-inexperienced (gray) cells. A dotted line shows the DC1 subset. (D) Heat map showing top 10 down- and upregulated genes in CAT-experienced over CAT-inexperienced DC1s. The heat map color scale depicts average log2 fold change. (E) Violin plots show the expression of Cldn1 and Cd36 by CAT-experienced (orange) and CAT-inexperienced (gray) DC1. All mice were bred on the B6 background. Littermates were used.

    Techniques Used: Isolation, Expressing



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    Claudin 1 is upregulated in CAT-experienced DC1s. (A) FACS gating strategy used to perform scRNAseq of thymic myeloid cells. Thymic cells were isolated from Foxn1 Cre R26 TdTOMATO mice, MACS-enriched for CD11c + and <t>CD11b</t> + cells, and sorted as either TdTOMATO + or TdTOMATO−CD45 + CD11c + /CD11b + cells. (B) UMAP of annotated thymic myeloid cells from scRNAseq excluding subsets shown in red in the legend of . Individual cell lineages are demarcated by dotted lines. DC = conventional DC; aDC = activated DC; Mac = macrophages; Mono = monocytes; prolif = proliferating. (C) UMAP of scRNAseq corresponding to projecting CAT-experienced (orange) and CAT-inexperienced (gray) cells. A dotted line shows the DC1 subset. (D) Heat map showing top 10 down- and upregulated genes in CAT-experienced over CAT-inexperienced DC1s. The heat map color scale depicts average log2 fold change. (E) Violin plots show the expression of Cldn1 and Cd36 by CAT-experienced (orange) and CAT-inexperienced (gray) DC1. All mice were bred on the B6 background. Littermates were used.
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    Claudin 1 is upregulated in CAT-experienced DC1s. (A) FACS gating strategy used to perform scRNAseq of thymic myeloid cells. Thymic cells were isolated from Foxn1 Cre R26 TdTOMATO mice, MACS-enriched for CD11c + and CD11b + cells, and sorted as either TdTOMATO + or TdTOMATO−CD45 + CD11c + /CD11b + cells. (B) UMAP of annotated thymic myeloid cells from scRNAseq excluding subsets shown in red in the legend of . Individual cell lineages are demarcated by dotted lines. DC = conventional DC; aDC = activated DC; Mac = macrophages; Mono = monocytes; prolif = proliferating. (C) UMAP of scRNAseq corresponding to projecting CAT-experienced (orange) and CAT-inexperienced (gray) cells. A dotted line shows the DC1 subset. (D) Heat map showing top 10 down- and upregulated genes in CAT-experienced over CAT-inexperienced DC1s. The heat map color scale depicts average log2 fold change. (E) Violin plots show the expression of Cldn1 and Cd36 by CAT-experienced (orange) and CAT-inexperienced (gray) DC1. All mice were bred on the B6 background. Littermates were used.

    Journal: The Journal of Experimental Medicine

    Article Title: Claudin 1–mediated positioning of DC1 to mTECs is essential for maintenance of central tolerance

    doi: 10.1084/jem.20250970

    Figure Lengend Snippet: Claudin 1 is upregulated in CAT-experienced DC1s. (A) FACS gating strategy used to perform scRNAseq of thymic myeloid cells. Thymic cells were isolated from Foxn1 Cre R26 TdTOMATO mice, MACS-enriched for CD11c + and CD11b + cells, and sorted as either TdTOMATO + or TdTOMATO−CD45 + CD11c + /CD11b + cells. (B) UMAP of annotated thymic myeloid cells from scRNAseq excluding subsets shown in red in the legend of . Individual cell lineages are demarcated by dotted lines. DC = conventional DC; aDC = activated DC; Mac = macrophages; Mono = monocytes; prolif = proliferating. (C) UMAP of scRNAseq corresponding to projecting CAT-experienced (orange) and CAT-inexperienced (gray) cells. A dotted line shows the DC1 subset. (D) Heat map showing top 10 down- and upregulated genes in CAT-experienced over CAT-inexperienced DC1s. The heat map color scale depicts average log2 fold change. (E) Violin plots show the expression of Cldn1 and Cd36 by CAT-experienced (orange) and CAT-inexperienced (gray) DC1. All mice were bred on the B6 background. Littermates were used.

    Article Snippet: Next, cells were stained with anti-biotin magnetic beads (Miltenyi) according to the manufacturer’s protocol and CD11c + and CD11b + cells were MACS-enriched using QuadroMACS (Miltenyi).

    Techniques: Isolation, Expressing